Clinical Benefits: Supports red blood cell formation and helps prevent megaloblastic anaemia Essential for neurological function and nerve tissue health Plays a key role in DNA synthesis and cell metabolism Supports cognitive function, concentration, and mood regulation Assists in the conversion of homocysteine, supporting cardiovascular health Helps address fatigue and weakness associated with B12 deficiency For patients presenting with deficiency, a loading protocol is typically recommended: weekly injections for three weeks, followed by monthly injections for three months, then a maintenance injection every three months

gentle warming and extended mixing may be required Do not use organic solvents (acetonitrile, DMSO) unless specifically validated for the peptide Solution is Cloudy or Precipitated Cloudiness may indicate peptide aggregation or poor solubility Try gentle warming to 37 C and slow cooling to room temperature If precipitation persists, the peptide may be degraded or the concentration too high Consider diluting to a lower concentration or using a different buffer (e.g., PBS with 0.1% BSA) Foam Formation Foam is caused by vigorous shaking or forceful injection of water Allow foam to settle naturally (do not shake to disperse) If foam persists, briefly centrifuge the vial at low speed (e.g., 500 g for 2 minutes) To prevent foam: inject water slowly down the vial wall
In addition to excessive intake of dietary supplements, parenteral administration of vitamin B12 may be another cause of excess, which is likely associated with an immune system response to produce anti-TC II antibodies
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